Advantages Of Serial Dilution Method

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Advantages Of Serial Dilution Method Pdf

A serial dilution is the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M ... Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10^0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10^0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.

What are the advantages of the serial dilution method for diluting cells? What are the possible sources of error w. To accomplish this, the serial dilution–agar plate technique is used. Briefly, this method involves serial dilution of a bacterial suspension in sterile water blanks, which serve as a diluent of known volume. Once diluted, the suspensions are placed on suitable nutrient media. The pour-plate technique is the procedure usually employed. Prepare the dilution of the test sample expected to contain between 30-300 CFU/mL. (Follow serial dilution technique) Inoculate labeled empty petri dish with specified mL (0.1 or 1.0 mL) of diluted specimen; Note: for the detail description regarding use of pipette, inoculation of sample, dilution technique etc, follow the reference 1.

In biology and medicine[edit]

In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results.^[1] Serial dilution is also a cheaper and simpler method for preparing cultures from a single cell than optical tweezers and micromanipulators.^[2]

In homeopathy[edit]

Serial dilution is one of the core foundational practices of homeopathy, with 'succussion', or shaking, occurring between each dilution. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain.^[3]^[4]

References[edit]

^K. R. Aneja. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. 69. ISBN81-224-1494-X
^Booth, C.; et al. (2006). Extremophiles. Methods in microbiology 35. Academic Press. p. 543. ISBN978-0-12-521536-7.
^Weissmann, Gerald (2006). 'Homeopathy: Holmes, Hogwarts, and the Prince of Wales'. The FASEB Journal. 20 (11): 1755–1758. doi:10.1096/fj.06-0901ufm. PMID16940145. Retrieved 2008-02-01.
^Ernst, Edzard (November 2005). 'Is homeopathy a clinically valuable approach?'. Trends in Pharmacological Sciences. 26 (11): 547–548. CiteSeerX10.1.1.385.5505. doi:10.1016/j.tips.2005.09.003. PMID16165225.

Michael L. Bishop, Edward P. Fody, Larry E. Schoeff. Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams & Wilkins, 2004, p. 24. ISBN0-7817-4611-6.